Key Publications

·    Lu M, Hui E, Brockhoff M, Trauble J, Fernandez-Villegas A, Burton O J, ... & Kaminski Schierle G. S. (2024). Graphene microelectrode arrays, 4D structured illumination microscopy, and a machine learning-based spike sorting algorithm permit the analysis of ultrastructural neuronal changes during neuronal signalling in a model of Niemann-Pick disease type C. bioRxiv, 2024-02.

 

 

·    Lu M, Christensen CN, Weber J, Konno T, Läubli N, Scherer K, Lapkin A, Lio P, Avezov E, Kaminski Schierle GS, Kaminski CF. 2023. ERnet: a tool for the semantic segmentation and quantitative analysis of endoplasmic reticulum topology. Nature Methods. Apr, 20(4):569-579. 

A SOTA deep-learning model to segment, classify and analyse the dynamic structures of the endoplasmic reticulum, the largest organelle within the cell.

 

 

·    Lu M, van Tartwijk FW, Lin JQ, Nijenhuis W, Parutto P, Fantham M, Christensen CN, Avezov E, Holt CE, Tunnacliffe A, Holcman D, Kapitein L, Kaminski Schierle GS, Kaminski CF. 2020. The structure and global distribution of the endoplasmic reticulum network are actively regulated by lysosomes. Science Advances. 16;6(51): eabc7209.  

This research was reported on the University of Cambridge website and social media:

“Driving force behind cellular ‘protein factories’ could have implications for neurodegenerative  disease”, front page of University of Cambridge website, 16th Dec, 2020.

 

 

·    Lu M, Williamson N, Mishra A, Michel CH, Kaminski CF, Tunnacliffe A. and Kaminski Schierle GS. 2019. Structural progression of amyloid-β Arctic mutant aggregation in cells revealed by multiparametric imaging. J. Biol. Chem. 294(5), pp.1478-1487. 

Selected as “the representative ‘Molecular Biophysics’ article of 2019” in the Journal of Biological  Chemistry. 

Cover story, designated as ‘Editors’ picks’ and highlighted with a preview “Capturing Aβ    aggregation in the cell” by Francesco Bemporad, Cristina Cecchi and Fabrizio Chiti in J. Biol. Chem.) 

“For the first time, the acceleration in Aβ42 aggregation induced by the Arctic mutation was monitored in cells, revealing a number of distinct morphologies that form sequentially. This approach will help discriminate the impacts of mutations on amyloid protein processing, Aβ aggregation propensity, and other mechanistic outcomes.” ——“Capturing Aβ aggregation in the cell” by Francesco Bemporad, Cristina Cecchi and Fabrizio Chiti, J. Biol. Chem.

Other Publications

·     Lu M, Williamson N, Mishra A, Michel CH, Kaminski CF, Tunnacliffe A. and Kaminski Schierle GS. 2019. Structural progression of amyloid-β Arctic mutant aggregation in cells revealed by multiparametric imaging. J. Biol. Chem. 294(5), pp.1478-1487.

 

·     Ward EN, Hecker L, Christensen CN, Lamb JR, Lu M, Mascheroni L, ... & Kaminski CF. 2022. Machine learning assisted interferometric structured illumination microscopy for dynamic biological imaging. Nature Comms., 13(1), 7836.

 

·     Christensen CN, Lu M, Ward EN, Lio P. and Kaminski CF. 2022. Spatio-temporal Vision Transformer for Super-resolution Microscopy. arXiv preprint arXiv:2203.00030.

 

·     Zhu GJ, 1, Azharuddin M, Bramanik B, Roberg K, D’arcy K, Lu M, Chen A, Zhuang S, Backer A, Liu XW, Chen RG, Slater NKH, Mazumder B, Kaminski CF, Kaminski Schierle GS, Hinkula J, and Patra. HK. Feasibility of coacervate nanostructure for instant drug nanoformulation. Under revision in ACS Applied Materials & Interfaces.

 

·     Parutto P, Heck J, Lu M, Kaminski CF, Avezov E, Heine M and Holcman D. 2021. High-throughput super-resolution single particle trajectory analysis reconstructs organelle dynamics and membrane re-organization. Cell Reports Methods. Available at SSRN 3985166.

 

·     Lu M, Ward E, van Tartwijk FW, Kaminski CF. 2021. Advances in the study of organelle interactions and their role in neurodegenerative diseases enabled by super-resolution microscopy. Neurobiology of Disease, p.105475.

 

·     Lu M, Mishra A, Boschetti C, Kaminski CF, Huang Z, Tunnacliffe A, Kaminski Schierle GS. 2021. Synthetic peptides derived from sea cucumber alleviate oxidative stress in neuroblastoma cells and improve survival in Caenorhabditis elegans exposed to neurotoxic paraquat. Oxidative Medicine and Cellular Longevity. 8842926.

 

·     Christensen C, Ward E, Lu M, Lio P, Kaminski CF. 2021. ML-SIM: Universal reconstruction of structured  illumination microscopy images using transfer learning. Biomedical Optics Express. 12 (5), 2720-2733.

 

·     Stephens A D, Lu M, Fernandez-Villegas A and Kaminski Schierle GS. 2020. Fast Purification of Recombinant Monomeric Amyloid-β from E. coli and Amyloid-β-mCherry Aggregates from Mammalian Cells. ACS Chemical Neuroscience 11 (20), 3204-3213.

 

·     Lautenschlaeger J, Wagner S, Stephens A, Fernández-Villegas A, Hockings C, Mishra A, Manton J, Fantham M, Lu M, Rees E, Kaminski CF, Kaminski Schierle GS. 2020. Intra-mitochondrial protein degradation is directly coupled to alpha-synuclein and Amyloid β 1-42 pathologies. J. Biol. Chem. May 8: jbc-RA119.

 

·     Lu M, Kaminski CF, Kaminski Schierle GS. 2020. Advanced fluorescence imaging of in situ protein aggregation. Physical Biology. Jan 8.

 

·     Stephens AD, Lu M, and Kaminski Schierle GS. 2019. Isolation and Imaging of His-and RFP-tagged Amyloid-like Proteins from Caenorhabditis elegans by TEM and SIM. Bio-protocol 9 (21): e3408.

 

·     Teplensky MH, Fantham M, Poudel C, Hockings C, Lu M, Guna A, et al. 2019. A highly porous metal-organic framework system to deliver payloads for gene knockdown. Chem, 5(11), 2926-2941.

 

·     Huang C, Wagner-Valladolid S, Stephens AD, Jung R, Poudel C, Sinnige T, Lechler MC, Schlörit N, Lu M, Laine RF, Michel CH et al. 2019. Intrinsically aggregation-prone proteins form amyloid-like aggregates and contribute to tissue aging in Caenorhabditis elegans. Elife. May 3;8:e43059.

 

·     Lu M, Banetta L, Young LJ, Smith EJ, Bates GP, Zaccone A, Kaminski Schierle GS, Tunnacliffe A, Kaminski CF. 2019. Live-cell super-resolution microscopy reveals a primary role for diffusion in polyglutamine-driven aggresome assembly. J. Biol. Chem. Jan 4;294 (1):257-68.

 

·     Chen WY, Young LJ, Lu M, Zaccone A, Ströhl F, Yu N, Kaminski Schierle GS, Kaminski CF. 2016. Fluorescence self-quenching from reporter dyes informs on the structural properties of amyloid clusters formed in vitro and in cells. Nano Lett. 17 (1), 143-149.

 

·     Lu M, Boschetti C, Tunnacliffe A. 2015. Long-term polyglutamine aggresome accumulation leads to DNA damage with p53-dependent cell-cycle arrest and steric interference of mitosis. J. Biol. Chem. 290, 27986–28000.

 

·     Lu M, Williamson N, Boschetti C, Ellis T, Yoshimi T, Tunnacliffe A. 2015. Expression-level dependent perturbation of cell proteostasis and nuclear morphology by aggregation-prone polyglutamine proteins. Biotechnol. Bioeng. 112, 1883–1892.